Posted on May 14, 2016
amoxicillin and lactobacillus
A recently published paper has shown that L. reuteri is effective in reducing gastrointestinal symptoms during H. pylori eradication therapy inchildren [29–33]. Amoxicillin and lactobacillus.
Middle East J. Agric. Res., 5(1): 10-18, 2015 4 plantarum to hydrochloric acid. In contrast, exposure to pH 2 eliminated more than 11.2 and 12.4 % forL. casei and L. plantarum, respectively during an incubation period of 2 h and then after 3 h elimination was more than 22.0 and 23.3 % for L. caseiand L. plantarum , respectively at the same pH. Acid tolerance of Lactobacillus strains was also reported by Dixit et al. (2013) who recorded that,the survival of L. acidophilus at pH 2.5 after 4 h was very significant with high acid tolerance. Srinu et al. (2013) showed that all the selectedlactic acid bacterial strains (L. plantarum and L. casei) were good survival abilities in the tested acidic pH range (1.5, 2.0, 3.0 and 3.5) andShivram and Vishwanath (2012) reported that, the isolated Lactobacillus strains were tolerable to pH 2 and 3. Also, Mourad and Nour-Eddine, (2006)reported that L. plantarum was survived at an incubation periods of 2 to 6 h at pH 2.0 and 3.0. Park et al. ( 2006) compared the pH resistance of thefour strains of L. acidophilus at pH 2, 3, 4, 5 and 7. Lactobacillus acidophilus isolated from rat and chicken showed little or no decrease in viablecell numbers for up to 240 min at pH 3, 4, 5 and 7. Jamaly et al. (2011) found L. plantarum able to tolerate 3 h of acid exposure with pH 2.0 and 3.0. Bile salt tolerance Bile is a result from a digestive secretion that can play a capital role in lipids emulsification and has the ability to affectthe phospholipids, cell membrane proteins and disrupt cellular homeostasis (Burns et al., 2008). Bile tolerance is one of the most crucial propertiesas it determines the ability of bacteria to survive in the small intestine, and consequently their capacity to play their functional role asprobiotics. The ability of Lactobacillus strains to grow in the presence of bile salts was studied by growing them in MRS broth supplemented withdifferent concentrations of bile salts (0.1 to 0.7%). Bile tolerance is an important characteristic of probiotic microorganisms. A concentration of0.3% of bile salts closely appropriates the bile level found in the gastrointestinal tract (Goldin and Gorbach 1992). Results from comparison ofdifferent cultures for bile salts tolerance are shown in Table (1). All strains exhibited considerable variations with regard to growth in controlbroth after 24 h. The optical densities of L acidophilus reached to 2.905, 2.900, 2.721, 2.740 and 2.626 at concentration 0, 0.1, 0.3, 0.5 and 0.7 %of bile salts, respectively. While, the corresponding percentage were 2.882, 2.748, 2.668, 2.573 and 2.476 for L. casei and it reached 2.905, 2.822,2.314, 2.250 and 2.229 for L. plantarum after 24 h. Table 1: Effect of bile salt concentration on growth of Lactobacillus strains at 370C.Bile salt concentration % 0 2 0.0 0.095 0.186 0.1 0.102 0.162 0.3 0.116 0.187 0.5 0.021 0.092 0.7 0.118 0.182 0.0 0.138 0.227 0.1 0.153 0.269 0.30.170 0.272 0.5 0.167 0.286 0.7 0.164 0.336 0.0 0.039 0.090 0.1 0.053 0.105 0.3 0.074 0.097 0.5 0.075 0.128 0.7 0.060 0.099 Surviving percentage(%) = (OD of bile salt /OD of control) x100 In another way, the obtained results also showed that three strains were observed resistantbile salt, corresponding to survival percentages ranging from 99.82, 93.66, 94.39 and 90.32 %, respectively, after 24 h incubation for L. acidophilusand resistance percentage of L. casei was 95.35, 92.57, 89.27 and 85.91 % at 0.1, 0.3, 0.5 and 0.7 % of bile salt, respectively after 24 h incubation.While, L. plantarum was also resists to bile salt and it was 97.14, 79.65, 77.45 and 76.72 % at 0.1, 0.3, 0.5 and 0.7 % of bile salt, respectively,after 24 h incubation. These results are approach with those of Jamaly et al. (2011), they reported that 10 out of 18 resistant Lactobacilli strainsto 0.3% Ox-bile (with resistance % ≥ 50) were obtained and identified as L. plantarum, L. paracasei and L. brevis. Kim et al. (2006) mentioned thatL. gasseri, L. acidophilus and L brevis grew in the presence of bile at 1.0%, during incubation for 24 h. The survival of Lactobacillus strains atbile concentrations of 0.5 and 1% were very similar. Lactobacillus gasseri showed more resistant than other strains. In the presence of 1% bile salts,L. gasseri was increased by 2.11 log cfu/ml for 24 h incubation. On the other hand, L. acidophilus showed a high degree of sensitivity, and wasincreased by 1.12 log cfu/ml with bile salts, while by 2.94 log cfu/ml without bile salts. Conway et al. (1987) suggested that, these differences inacid and bile tolerance of strains from species might be due to difference in the cell wall structure. Ruiz et al. (2013) reported that Lactobacillusand Bifidobacterium display a variety of proteins devoted to the efflux of bile salts or Strains Time (h) Surviving (%) after 24 h 100.00 99.82 93.6694.32 90.39 100.00 95.35 92.57 89.27 85.91 100.00 97.14 79.65 77.45 76.72 4 6 8 24 2.905 2.900 2.721 2.740 2.626 2.882 2.748 2.668 2.573 2.476 2.9052.822 2.314 2.250 2.229 L. acidophilus 0.522 0.439 0.389 0.294 0.349 1.282 1.333 1.215 1.061 1.156 0.265 0.278 0.246 0.232 0.242 1.325 1.140 0.7970.702 0.612 2.317 2.272 1.931 1.855 2.015 0.758 0.716 0.584 0.492 0.497 2.014 1.860 1.463 1.368 1.262 2.803 2.426 2.115 2.058 2.180 1.627 1.487 1.1490.923 0.848 L. casei L. plantarumi
Amoxicillin and lactobacillus - readmore
Readmore amoxicillin and lactobacillus
Ninety consecutive H. pylori-positive patients were enrolled from November 2007 to June 2008. Patients were considered eligible to enter the study ifthey were between 18 and 65 years old, affected by gastric H. pylori infection as confirmed by a 13C-urea breath test, submitted to a previousunsuccessful anti-H. pylori antibiotic treatment. Exclusion criteria were recent (within the previous 3 months) use of antimicrobial agents, bismuthcompounds, PPI and H2 receptor antagonists, laxatives, antidiarrheal, other probiotic preparations, alcohol, or drug abuse. Patients with majorconcomitant diseases including psychiatric disorders and pregnant or lactating women were also excluded from the study. All patients signed a writteninformed consent. The study was approved by our Ethical Committee.
Feb 5, 2016 . How to Take Acidophilus With Antibiotics. Your digestive tract has a delicate balance of "good" beneficial bacteria and "bad" harmful bacteria.
Introduction. Helicobacter pylori eradication therapy has the potential burden of antibiotic-associated gastrointestinal (GI) side effects. Theoccurrence of side effects is among the major drawbacks of such regimens. GI manifestations may be related to alterations in the intestinalmicroflora. Probiotics can prevent or reduce antibiotic-associated side effects and have an inhibitory effect on H. pylori. Methods. To define theefficacy of Lactobacillus reuteri supplementation in H. pylori eradication and in preventing GI-associated side effects during a second-linelevofloxacin triple therapy. 90 H. pylori-positive patients receive for 7 days a second-line triple therapy with esomeprazole, levofloxacin, andamoxicillin with L. reuteri for 14 days (group 1) and without probiotic supplementation (group 2). Each subject received a validated questionnaire torecord symptoms everyday for 4 weeks from the start of therapy. H. pylori status and side effects were assessed 6 weeks after treatment. Results. Theeradication rate was significantly influenced by probiotic supplementation with L. reuteri (group 1: 36/45, 80%; group 2: 28/45 62%; ). The incidence of nausea and diarrhoea in group 1 was significantly lower than that in group 2.Conclusion. In H. pylori-positive subjects L. reuteri supplementation increases the eradication rate while reducing the incidence of the most commonside effects associated with antibiotic therapy in second-line treatment.
The gastrointestinal tract microflora of poultry is a mixture of bacteria, fungi and protozoa but bacteria are the predominant microorganisms. Broilerchicken diets containing corn, sorghum, barley, oats or rye had various effects as corn- and sorghum-based diets increased numbers of Enterococcus,barley-based diet increased numbers of Lactobacillus, oats-based diet enhanced growth of Escherichia and Lactococcus and rye-based diet increased thenumber of Streptococcus in broiler chickens.
The microflora of the crop consists of large numbers of lactobacilli and smaller numbers of coliforms and streptococci. The lactobacilli remaindominant throughout the small intestine. It is only in the caeca where different nutritional conditions exist and residence time is longerthat the strict anaerobes become the dominant components of the microflora (Fuller, 2001). A key requirement for probiotic strains is thatthey should not carry transmissible antibiotic resistance genes. Ingestion of bacteria carrying such genes is undesirable as horizontal genetransfer to recipient bacteria in the gut could lead to the development of new antibiotic-resistant pathogens (Zhou et al., 2005).
Lactobacillus jonshonii LC2 is susceptible to penicillin, amoxicillin and clindomycin and resistant to the others antibiotics. Lactobacillusgallinarum LC3 is resistant to all antibiotics tested except Clindomycin and fusidic acid. Lactobacillus crispatus LC4 is susceptible both toamoxicillin and spiramycin. Lactobacillus aviarus LC5 is resistant to all antibiotics tested (Table 2 and 3).
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Several mechanisms of antimicrobial resistance are readily spread to a variety of bacterial genera. First, the organism may acquire genes encodingenzymes such as lactamases that destroy the antibacterial agent before it can have an effect. Second, bacteria may acquire efflux pumps that extrudethe antibacterial agent from the cell before it can reach its target site and exert its effect.buy.|)
As regards antibiotic resistance rate a high resistance versus metronidazole and clarithromycin was reported in our country.
Third, bacteria may acquire several genes for a metabolic pathway which ultimately produces altered bacterial cell walls that no longer containthe binding site of the antimicrobial agent or bacteria may acquire mutations that limit access of antimicrobial agents to the intracellulartarget site via down regulation of porin genes (Tenover, 2006). Lactic acid bacteria may act as reservoirs of antibiotic resistance genes thatcan be transferred via the food chain or within the gastrointestinal tract to pathogenic bacteria (Egervarn, 2009).
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In poultry farming as well as with other intensively reared animals, antibiotics may be administered, though feed or drinking water to whole focksrather than to individual animals. In the European Community (EC), the water or feed-based administration of antimicrobials to animals (at lower dosesthan those employed for therapeutic purposes) to enhance animal growth has been completely banned since, January 2006.