Posted on May 14, 2016






amoxicillin and lactobacillus

Pylopass™ (English Version) amoxicillin and lactobacillus.

A recently published paper has shown that L. reuteri is effective in reducing gastrointestinal symptoms during H. pylori eradication therapy inchildren [29–33]. Amoxicillin and lactobacillus.

Middle East J. Agric. Res., 5(1): 10-18, 2015 4 plantarum to hydrochloric acid. In contrast, exposure to pH 2 eliminated more than 11.2 and 12.4 % forL. casei and L. plantarum, respectively during an incubation period of 2 h and then after 3 h elimination was more than 22.0 and 23.3 % for L. caseiand L. plantarum , respectively at the same pH. Acid tolerance of Lactobacillus strains was also reported by Dixit et al. (2013) who recorded that,the survival of L. acidophilus at pH 2.5 after 4 h was very significant with high acid tolerance. Srinu et al. (2013) showed that all the selectedlactic acid bacterial strains (L. plantarum and L. casei) were good survival abilities in the tested acidic pH range (1.5, 2.0, 3.0 and 3.5) andShivram and Vishwanath (2012) reported that, the isolated Lactobacillus strains were tolerable to pH 2 and 3. Also, Mourad and Nour-Eddine, (2006)reported that L. plantarum was survived at an incubation periods of 2 to 6 h at pH 2.0 and 3.0. Park et al. ( 2006) compared the pH resistance of thefour strains of L. acidophilus at pH 2, 3, 4, 5 and 7. Lactobacillus acidophilus isolated from rat and chicken showed little or no decrease in viablecell numbers for up to 240 min at pH 3, 4, 5 and 7. Jamaly et al. (2011) found L. plantarum able to tolerate 3 h of acid exposure with pH 2.0 and 3.0. Bile salt tolerance Bile is a result from a digestive secretion that can play a capital role in lipids emulsification and has the ability to affectthe phospholipids, cell membrane proteins and disrupt cellular homeostasis (Burns et al., 2008). Bile tolerance is one of the most crucial propertiesas it determines the ability of bacteria to survive in the small intestine, and consequently their capacity to play their functional role asprobiotics. The ability of Lactobacillus strains to grow in the presence of bile salts was studied by growing them in MRS broth supplemented withdifferent concentrations of bile salts (0.1 to 0.7%). Bile tolerance is an important characteristic of probiotic microorganisms. A concentration of0.3% of bile salts closely appropriates the bile level found in the gastrointestinal tract (Goldin and Gorbach 1992). Results from comparison ofdifferent cultures for bile salts tolerance are shown in Table (1). All strains exhibited considerable variations with regard to growth in controlbroth after 24 h. The optical densities of L acidophilus reached to 2.905, 2.900, 2.721, 2.740 and 2.626 at concentration 0, 0.1, 0.3, 0.5 and 0.7 %of bile salts, respectively. While, the corresponding percentage were 2.882, 2.748, 2.668, 2.573 and 2.476 for L. casei and it reached 2.905, 2.822,2.314, 2.250 and 2.229 for L. plantarum after 24 h. Table 1: Effect of bile salt concentration on growth of Lactobacillus strains at 370C.Bile salt concentration % 0 2 0.0 0.095 0.186 0.1 0.102 0.162 0.3 0.116 0.187 0.5 0.021 0.092 0.7 0.118 0.182 0.0 0.138 0.227 0.1 0.153 0.269 0.30.170 0.272 0.5 0.167 0.286 0.7 0.164 0.336 0.0 0.039 0.090 0.1 0.053 0.105 0.3 0.074 0.097 0.5 0.075 0.128 0.7 0.060 0.099 Surviving percentage(%) = (OD of bile salt /OD of control) x100 In another way, the obtained results also showed that three strains were observed resistantbile salt, corresponding to survival percentages ranging from 99.82, 93.66, 94.39 and 90.32 %, respectively, after 24 h incubation for L. acidophilusand resistance percentage of L. casei was 95.35, 92.57, 89.27 and 85.91 % at 0.1, 0.3, 0.5 and 0.7 % of bile salt, respectively after 24 h incubation.While, L. plantarum was also resists to bile salt and it was 97.14, 79.65, 77.45 and 76.72 % at 0.1, 0.3, 0.5 and 0.7 % of bile salt, respectively,after 24 h incubation. These results are approach with those of Jamaly et al. (2011), they reported that 10 out of 18 resistant Lactobacilli strainsto 0.3% Ox-bile (with resistance % ≥ 50) were obtained and identified as L. plantarum, L. paracasei and L. brevis. Kim et al. (2006) mentioned thatL. gasseri, L. acidophilus and L brevis grew in the presence of bile at 1.0%, during incubation for 24 h. The survival of Lactobacillus strains atbile concentrations of 0.5 and 1% were very similar. Lactobacillus gasseri showed more resistant than other strains. In the presence of 1% bile salts,L. gasseri was increased by 2.11 log cfu/ml for 24 h incubation. On the other hand, L. acidophilus showed a high degree of sensitivity, and wasincreased by 1.12 log cfu/ml with bile salts, while by 2.94 log cfu/ml without bile salts. Conway et al. (1987) suggested that, these differences inacid and bile tolerance of strains from species might be due to difference in the cell wall structure. Ruiz et al. (2013) reported that Lactobacillusand Bifidobacterium display a variety of proteins devoted to the efflux of bile salts or Strains Time (h) Surviving (%) after 24 h 100.00 99.82 93.6694.32 90.39 100.00 95.35 92.57 89.27 85.91 100.00 97.14 79.65 77.45 76.72 4 6 8 24 2.905 2.900 2.721 2.740 2.626 2.882 2.748 2.668 2.573 2.476 2.9052.822 2.314 2.250 2.229 L. acidophilus 0.522 0.439 0.389 0.294 0.349 1.282 1.333 1.215 1.061 1.156 0.265 0.278 0.246 0.232 0.242 1.325 1.140 0.7970.702 0.612 2.317 2.272 1.931 1.855 2.015 0.758 0.716 0.584 0.492 0.497 2.014 1.860 1.463 1.368 1.262 2.803 2.426 2.115 2.058 2.180 1.627 1.487 1.1490.923 0.848 L. casei L. plantarumi



Amoxicillin and lactobacillus - readmore